北京生命科学研究所转基因动物中心为研究所各实验室提供基因敲除、转基因小鼠制备服务。同时中心也会根据实验室的特殊需要，研发新的技术和方法用于动物疾病模型的建立。转基因动物中心主要提供以下的服务：

一. 转基因小鼠的制备：

1. 原核显微注射法

2. 利用转座子系统胞质显微注射法

二. 利用胚胎干细胞进行基因敲除/敲入小鼠的制备：

1. 打靶载体的构建

2. 胚胎干细胞打靶

3. 囊胚显微注射

三. 利用TALEN或Cas9/sgRNA技术直接胚胎注射法制备基因敲除/敲入小鼠：

1. TALEN或Cas9/sgRNA设计及载体构建

2. TALEN或Cas9/sgRNA mRNA体外转录

3. TALEN或Cas9/sgRNA mRNA受精卵显微注射

四. 小鼠品系资源的保种：

1. 精子冻存及ICSI法复苏

2. 胚胎冻存及复苏

五. 无特异性病原体小鼠品系的重建

六. 其他：如载体设计，基因型和表型分析的咨询等。

Transgenic Animal Center of National Institute of Biological Sciences, Beijing (TAC) is dedicated to provide services related to making genetically engineered mice. We are happy to provide lab-project orientated service, such as to test new techniques to make mouse models for human diseases. We are providing the following services:

1. Transgenic mice production:

Pronuclear injection

Cytoplasm injection with transposon

2. Knockout/knockin mice production using Esc:

Targeting vector construction

ES cell targeting

Blastocyst injection

3. Knockout/knockin mice production via zygotes injection of TALEN or Cas9/sgRNA:

TALEN or sgRNA construction

TALEN or sgRNA mRNA IVT

Zygotes injection of TALEN or Cas9/sgRNA mRNA

4. The preservation of mice strains:

Sperm cryopreservation and restoration via ICSI

Embryo cryopreservation and restoration

5. SPF Mice Rederivation.

6. Others: Vector Designing, Genotyping, and Consulting on Phenotype Characterizing.