邓兴旺 博士

北京生命科学研究所第一任共同所长

北京生命科学研究所资深研究员

Xing Wang Deng, Ph.D.

Co-Director and Investigator, NIBS, Beijing

教育经历

Education

1982年 北京大学生物系植物生理生化专业学士

B.S., Plant Biochemistry and Physiology, Biology, Department ofBiology, Beijing University, China

1985年 北京大学生物系硕士

M.S., Biophysics and Biochemistry of Photosynthesis, Departmentof Biology, Beijing University, China

1989年 加利福尼亚大学伯克利分校植物生理及分子生物学博士

Ph.D., Molecular and Physiological Plant Biology, University ofCalifornia at Berkeley

工作经历

Professional Experience

2003-2009年 北京生命科学研究所所长及资深研究员

Co-Director and Investigator, National Institute of Biological Sciences, Beijing, China

2003年- 耶鲁大学植物生物学冠名教授

Named Daniel C. Eaton Professor of Plant Biology, YaleUniversity

2001年- 耶鲁大学分子与细胞发育生物学系教授

Professor, Department of MCD Biology, Yale University (approved and official starting date July 1, 2001)

2000年- 北大-耶鲁植物分子遗传学及农业生物技术联合中心主任

Director, the Peking-Yale joint center for Plant Molecular Genetics and Agribiotechnology and Cheung Kong Professor ofPeking University

1995-2001年 耶鲁大学分子与细胞发育生物学系副教授

Associate Professor, Department of MCD Biology, Yale University

1992-1995年 耶鲁大学分子与细胞发育生物学助理教授

Assistant Professor, Department of Biology, Yale University

1989-1991年 博士后研究—拟南芥光形态建成的分子生物学和遗传学分析，及燕麦PhyA基因核蛋白—启动子相互作用的体外Dnase I指纹分析

Post-doctoral research---Molecular and genetic analysis ofphotomorphogenesis in Arabidopsis thaliana, and in vitro DNase I footprinting analysis of nuclear protein-promoter interactions of Oat PhyA gene

研究概述

Research Description

我们实验室研究兴趣主要集中在两个领域：其中一个领域主要是研究光调控的拟南芥幼苗发育过程的分子和生化机制。我们实验室在研究参与光调控的拟南芥发育过程的遗传通路时，找到了12个多效性COP/DET/FUS位点，确定它们介导了光对拟南芥幼苗发育过程的调节。其中，COP1是拟南芥光形态建成的关键的抑制因子，暗中在细胞核内作为E3通过26S蛋白体降解促进光形态建成的转录因子，而在光下COP1活性被抑制且在核内的丰度降低；另外一个基因COP10编码一个类似E2的蛋白；其余的多数基因编码一个高度保守的多亚基蛋白COP9复合体（COP9 signalosome）的不同亚基，COP9复合体是一个新的E3连接酶的调节因子，可以促进NEDD8/RUB1从特定的E3连接酶上解离下来。COP9复合体是细胞对外界刺激或胁迫产生反应的新的调节成分。目前，本实验室正在通过分子遗传和基因组学的方法，进一步深入地研究这类在所有多细胞有机体中保守的细胞调节复合体的功能。

我们实验室另外一个研究领域是在水稻中从基因组水平探讨DNA甲基化和chromatin的修饰和结构，我们已经建立了一系列的实验技术平台，包括全基因组表达芯片，高密度寡核苷酸tilling芯片，以及ChIP-chip分析方法，可用于研究全基因组水平的基因表达以及DNA和组蛋白修饰的模式，我们的目的是寻找水稻的栽培过程和杂种优势形成的分子及遗传基础。

My research at NIBS is focused on two areas. One is the molecular and biochemical basis for light control of Arabidopsis development. Our studies have revealed a dozen pleiotropic COP/DET/FUS loci responsible for mediating light control of seedling development. These include COP1, which functions as the master repressor of photomorphogenic development and acts within the nucleus as an E3 ligase by directly targeting photomorphogenesis-promoting transcription factors for degradation by the 26S proteasome in darkness. Light inactivates COP1 and causes a reduction in its nuclear abundance. Another gene, COP10, encodes a probable E2 component. Most remaining genes encode subunits of a highly conserved multi-subunit protein complex, the COP9 signalosome, a novel regulator of the E3 ligases that promotes de-conjugation of NEDD8/RUB1 from certain E3 ligases. This group of regulatory proteins enables the cell to respond to external stimuli or stresses. Currently, we are applying both molecular genetic and genomic approaches to further analyze the components of this novel cellular machinery, which is conserved among all multicellular organisms. Our second area of research is the genome-wide organization of DNA elements and chromatin in rice. We have constructed a series of technology platforms, including a whole genome expression microarray, a high density oligomer tiling microarray, and a ChIP-chip assay, to define genome wide gene expression profiles as well as DNA and histone modification patterns. Our goal here is to search for the genomic basis for rice domestication and heterosis.

发表文章

Publications

1.Chen H., Shen Y., Tang X., Yu L., Wang J., Guo L., Zhang Y., Zhang H., Feng S., Strickland E., Zheng N. and Deng X.W.. Arabidopsis Cullin 4 Forms an E3 Ubiquitin Ligase with RBX1 and the CDD Complex in Mediating Light Control of Development. The Plant Cell. 2006 Aug.; 18(8).

2.Guo L., Yin B., Zhou J., Li X., Deng X.W.. Development of rabbit monoclonal and polyclonal antibodies for detection of site-specific histone modifications and their application in analyzing overall modification levels.Cell Research.2006; 16: 519-527.

3.Han Z., Guo L., Wang H., Shen Y., Deng X.W., Chai J.. Structural Basis for the Specific Recognition of Methylated Histone H3 Lysine 4 by the WD-40 Protein WDR5. Mol Cell. 2006 Apr.;22(1):137-44.

4.Li J., Zhu S., Song X., Shen Y., Chen H., Yu J., Yi K., Liu Y., Karplus V.J., Wu P., Deng X.W.. A Rice Glutamate Receptor-Like Gene Is Critical for the Division and Survival of Individual Cells in the Root Apical Meristem. Plant Cell. 2006 Feb.; 18(2):340-9. Epub 2005 Dec 23.

5.Li L., Wang X., Stolc V., Li X., Zhang D., Su N., Tongprasit W., Li S., Cheng Z., Wang J., Deng X.W.. Genome-wide transcription analyses in rice using tiling microarrays. Nature Genetics. 2006 Jan.; 38: 124 – 129. Epub Dec 20.

6.Stolc V., Li L., Wang X., Li X., Su N., Tongprasit W., Han B., Xue Y., Li J., Snyder M., Gerstein M., Wang J. and Deng X.W.. A pilot study of transcription unit analysis in rice using oligonucleotide tiling-path microarray. Plant Mol Bio. 2005; 59(1):137-149.

7.Ma L., Chen C., Liu X.，Jiao Y., Su N., Li L., Wang X., Cao M., Sun N., Zhang X., Bao J., Li J., Pedersen S., Bolund L., Zhao H., Yuan L., Wong G.K.S., Wang J., Deng X.W., Wang J.. A microarray analysis of the rice transcriptome and its comparison to Arabidopsis. Genome Research. 2005; 15(9):1274-1283.

8.Li L., Wang X., Xia M., Stolc V., Su N., Peng Z., Li S., Wang J., Wang X.,Deng X.W.. Tiling microarray analysis of rice chromosome 10 to identify the transcriptome and relate its expression to chromosomal architecture. Genome Biol. 2005; 6(6):R52.

9.Jiao Y., Jia P., Wang X., Su N., Yu S., Zhang D., Ma L., Feng Q., Jin Z., Li L., Xue Y., Cheng Z., Zhao H., Han B., Deng X.W.. A Tiling Microarray Expression Analysis of Rice Chromosome 4 Suggests a Chromosome-Level Regulation of Transcription. Plant Cell. 2005; 17(6):1641-57.

10.Li L., Wang X., Li X., Su N., Stolc V., Han B., Li J., Xue Y., Wang J., Deng X.W.. Toward genome-wide transcriptional analysis in rice using MAS oligonucleotide tiling-path microarrays. Rice Is Life: Scientific Perspectives For The 21st Century. 2004.