王晓晨 博士

北京生命科学研究所高级研究员

Xiaochen Wang, Ph.D. Associate Investigator, NIBS, Beijing, China

Phone：010-80726688

Fax： 010-80726689

E-mail：wangxiaochen@nibs.ac.cn

教育经历

Education

1999年 北京大学生命科学学院植物基因工程和蛋白质工程国家重点实验室博士

Ph.D., National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, China

1992年 山东大学微生物学系微生物学士

B.S. Microbiology, Department of Microbiology, Shandong University,China

工作经历

Professional Experience

2011年- 北京生命科学研究所高级研究员

Associate Investigator, National Institute of Biological Sciences, Beijing, China  

2006-2011年 北京生命科学研究所研究员

Assistant Investigator, National Institute of Biological Sciences,Beijing, China

1999-2006年 美国科罗拉多大学分子与细胞发育生物学系博士后

Postdoctoral research associate, Department of MCD Biology,University of Colorado, Boulder, USA

1998-1999年  比利时根特大学/佛兰德斯生物科技研究所访问学生

Visiting student, Laboratory of Genetics, Gent University/VIB,Belgium

研究概述

Research Description

凋亡细胞的吞噬及降解过程是整个细胞凋亡程序中不可缺少的一环，它在组织重塑，炎症抑制和免疫应答调控中起着重要的作用。对死细胞清除的障碍会引起诸如哮喘、类风湿性关节炎和狼疮等炎症疾病和自身免疫紊乱。

利用秀丽隐杆线虫作为模式动物，我们实验室运用遗传学，细胞生物学和生化的方法来研究凋亡细胞清除的分子机制。我们运用特异的正向遗传学及反向遗传学筛选手段寻找参与凋亡细胞清除过程的新基因，并对所鉴定的相关基因展开凋亡细胞吞噬，降解过程调节机制的研究，包括凋亡细胞信号的展示，识别，凋亡细胞的吞噬和降解。

Phagocytosis of apoptotic cells is an integral part of the cell death program and an important event in tissue remodeling, suppression of inflammation as well as regulation of immune response. Defects in this process contribute to inflammatory diseases and autoimmune disorders.

Using Caenorhabditis elegans as a model system, we take combinatory approaches of genetics, cell biology and biochemistry to identify new genes that control various events of cell corpse clearance including apoptotic cell recognition, internalization and degradation. The characterization and mechanistic studies of these novel players will help us to understand how phagocytosis of apoptotic cell is regulated in worm and in higher organisms like human being.

部分发表文章

Selected Publications

1. Wu, Y.W., Cheng, S.Y., Zhao, H.Y., Zou, W., Yoshina, S., Mitani, S., Zhang, H., *Wang, X.C. (2014) PI3P phosphatase activity is required for autophagosome maturation and autolysosome formation. EMBO Rep.15(9):973-81.2014/09
2. Wang H, Lu Q, Cheng S, *Wang X, *Zhang H(2013). Autophagy activity contributes to programmed cell death in Caenorhabditis elegans. Autophagy. 9(12):1975-82
3. Cheng S , Wu Y , Lu Q , Yan J, Zhang H， *Wang X(2013).Autophagy genes coordinate with the class II PI3 kinase PIKI-1 to regulate apoptotic cell clearance in C. elegans. Autophagy .9(12):2022-32, 2013/12
4. Li X, Chen B, Yoshina S, Cai T, Yang F, Mitani S, *Wang X(2013).Inactivation of C. elegans aminopeptidase DNPP-1 restores endocytic sorting and recycling in tat-1 mutants，Mol Biol Cell. 24(8):1163-75.
5. Huang J, Wang H, Chen Y, Wang X*, and Zhang H*(2012). Residual body removal during spermatogenesis in C. elegans requires genes that mediate cell corpse clearance. Development 139(24), 4613-4622. (*Co-corresponding author)
6. Zhang Y, Wang H, Kage-Nakadai E, Mitani S, and Wang X* (2012). C. elegans secreted lipid-binding protein NRF-5 mediates PS appearance on phagocytes for cell corpse engulfment. Curr Biol 22(14), 1276-1284.
7. Liu B, Du H, Rutkowski R, Gartner A, and Wang X* (2012). LAAT-1 is the lysosomal lysine/arginine transporter that maintains amino acid homeostasis.Science337, 351-354.
8. Kang Y, Zhao D, Liang H, Liu B, Zhang Y, Liu Q, Wang X*, and Liu Y* (2012). Structural study of TTR-52 reveals the mechanism by which a bridging molecule mediates apoptotic cell engulfment. Genes Dev. 26(12), 1339-1350. (*Co-corresponding author)
9. Li W, Zou W, Yang Y, Chai Y, Chen B, Cheng S, Tian D, Wang X*, Vale RD*, and Ou G* (2012). Autophagy genes function sequentially to promote apoptotic cell corpse degradation in the engulfing cell. J Cell Biol 197(1), 27-35. (Co-corresponding author)
10. Wu YC, Wang X and Xue D (2012). Methods for Studying Programmed Cell Death inC. elegans. in CAENORHABDITIS ELEGANS: CELL BIOLOGY AND PHYSIOLOGY, SECOND EDITION, ELSEVIER ACADEMIC PRESS INC, USA, 297-320.
11. Guo P, and Wang X* (2010). Rab GTPases act in sequential steps to regulate phagolysosome formation. Small GTPases 1(3), 1-4 (Invited Extra view).
12. Chen B, Jiang Y, Zeng S, Yan J, Li X, Zhang Y, Zou W, and Wang X* (2010). Endocytic sorting and recycling require membrane phosphatidylserine asymmetry maintained by TAT-1/CHAT-1. PLoS Genet 6(12), e1001235.
13. Guo P, Hu T, Zhang J, Jiang S, and Wang X* (2010). Sequential action of Caenorhabditis elegans Rab GTPases regulates phagolysosome formation during apoptotic cell degradation. Proc Natl Acad Sci U S A 107, 18016-18021.
14. Wang X*, Li W, Zhao D, Liu B, Shi Y, Chen B, Yang H, Guo P, Geng X, Shang Z,Peden E, Kage-Nakadai E, Mitani S, and Xue D* (2010). Caenorhabditis eleganstransthyretin-like protein TTR-52 mediates recognition of apoptotic cells by the CED-1 phagocyte receptor. Nat Cell Biol 12, 655-664. (Co-corresponding author)
15. Tian Y, Li Z, Hu W, Ren, H, Tian, E, Zhao Y, Lu Q, Huang X, Yang P, Li X,Wang X, Kovacs A L, Yu L and Zhang H (2010) C. elegans Screen Identifies Autophagy Genes Specific to Multicellular Organisms. Cell 141(6), 1042-1055.
16. Zou W, Lu Q., Zhao D, Li W, Mapes J, Xie Y, and Wang X* (2009).Caenorhabditis elegans myotubularin MTM-1 negatively regulates the engulfment of apoptotic cells. PLoS Genet 5, e1000679.
17. Li W, Zou W, Zhao D, Yan J, Zhu Z, Lu J, and Wang X* (2009). C. elegans Rab GTPase activating protein TBC-2 promotes cell corpse degradation by regulating the small GTPase RAB-5. Development 136, 2445-2455.
18. Lu Q, Zhang Y, Hu T, Guo P, Li W, and Wang X* (2008). C. elegans Rab GTPase 2 is required for the degradation of apoptotic cells. Development 135, 1069-1080.