何万中博士 - 科学研究 - 北京生命科学研究所

何万中博士

曾任北京生命科学研究所研究员

Former Assistant Investigator, NIBS, Beijing，China

教育经历

Education

1998年	中国科学院物理研究所物理学博士学位
	Ph.D. Physics, Institute of Physics, Chinese Academy of Sciences, Beijing, China
1994年	中国地质大学(北京)矿物学硕士学位
	M.S. Mineralogy, China University of Geosciences,Beijing, China
1991年	成都理工大学地质学学士学位
	B.S. Geology, Chengdu University of Technology,Chengdu, China

工作经历

Professional Experience

2010-	北京生命科学研究所研究员
	Assistant Investigator, National Institute of Biological Sciences, Beijing, China
2007-2010	新加坡国立大学生物科学系助理教授
	Assistant Professor, Department of Biological Sciences,National University of Singapore, Singapore
2004-2007	美国加州理工学院生物分部博士后
	Postdoctoral research with Dr. Pamela J. Bjorkman, Division of Biology, HHMI & California Institute of Technology, California, USA
1999-2004	美国纽约大学医学中心Skirball分子生物医学研究所博士后
	Postdoctoral research with Dr. David L. Stokes, Skirball Institute of Biomolecular Medicine, New York University Medical Center, New York, USA
1998-1999	西班牙CSIC国家生物技术中心博士后
	Postdoctoral research with Dr. Jose-Maria Carazo,Centro Nacional de Biotecnologia -C.S.I.C., Madrid,Spain

研究概述

本实验室致力于细胞电子断层成像技术的开拓及其在生物医学研究中的应用。细胞电子断层成像技术是一项新兴的生物电镜三维成像技术，它可以在纳米尺度解析瞬时冷冻固定的细胞中的精细结构、动态事件及分子定位，它有机地整合了生物细胞组织样品的超快速冷冻固定、冰冻切片、低温去水固定、基因技术、蛋白质及基因的标记识别、冰冻电镜成像和计算机图像处理等技术（见图1）。结合我们最新开发的蛋白质、基因标记的电镜识别定位技术，本实验室将开展一系列生物医学相关课题的细胞电子断层成像技术研究。目前本实验室的主要研究方向如下：

1.	生理状态下活体细胞中的蛋白质、基因的标记及相应的电镜检测定位技术
2.	瞬时捕捉生物体中动态分子事件和纳米尺度解析细胞的精细结构及复合体的组装过程
3.	解析疾病状态下（如病原体和宿主细胞的相互作用过程中的）动态分子事件及相应细胞精细结构的变化
4.	纳米尺度精确定位跟踪某些关键分子在胚胎发育及干细胞分化过程中的时间空间分布

Research Description

Our major interest is focused on the development of cellular electron tomography platform technology and its applications in biomedical research. Cellular electron tomography is an emerging three-dimensional electron microscopy bio-imaging technology, which can be broadly used to dissect the cellular ultrastructures, dynamic events, and precisely locating individual molecule in the rapid-frozen cells at nanometer resolution. Cellular electron tomography platform technology involves rapid-freezing technique, frozen-hydrated thin sectioning technique, freeze-substitution fixation, gene technology, protein labeling / genetic tagging and the corresponding detection techniques, cryo-electron microscopy and computer imaging processing techniques. We will initiate a series of biomedical-related cellular electron tomography projects with our newly developed protein labeling/genetic tagging and the corresponding detection techniques. Our current research will focus on the following areas:

1.	Developing novel in vivo protein labeling/genetic tagging and the corresponding electron microscopy detection technologies;
2.	Dissecting the dynamic molecular events, cellular ultrastructures and the assembling processes of large protein complexes in rapid-immobilized cells;
3.	Untangling the dynamic molecular events and the ultrastructures changes that involve in diseases, such as host-microbe interactions;
4.	Tracing the precise temporal-spatial distributions of the key proteins involved in embryonic development and stem cell differentiation processes.

发表文章

Publications

1.	Wanzhong He and Jose-Jesus Fernandez, (January 2010) Electron Tomography. In: ENCYCLOPEDIA OF LIFE SCIENCES 2010, John Wiley & Sons, Ltd: Chichester http://www.els.net/ [DOI: 10.1002/9780470015902.a0021877]. (Invited review)
2.	Wanzhong He, Mark S. Ladinsky, Kathryn E. Huey Tubman, Grant Jensen, J. Richard McIntosh, Pamela J. Bjorkman , FcRn-Mediated antibody transport across epithelial cells revealed by electron tomography, Nature, 2008 Sep 25;455(7212):542-546.
3.	Morphew M, He W, Bjorkman PJ, McIntosh JR. Silver enhancement of Nanogold particles during freeze substitution for electron microscopy. J Microsc. 2008 May;230(Pt 2):263-7.
4.	Wanzhong He, Christine Kivork, Suman Machinani, Mary K. Morphew, Anna M. Gail, Devin B. Tesar, Noreen E. Tiangco, J. Richard McIntosh, Pamela J. Bjorkman, Freeze Substitution-based gold-enlarging technique for EM studies of endocytosed nanogold-labeled ligands, J Struct Biol. 2007 Oct;160(1):103-13.
5.	Wanzhong He, Pamela Cowin, David L. Stokes, Untangling Desmosomal Knots with Electron Tomography, Science, 2003 Oct 3;302(5642):109-113.
6.	Chen Xu, William J. Rice, Wanzhong He and David L. Stokes, A Structural Model for the Catalytic Cycle of Ca2+-ATPase. Journal of Molecular Biology, vol.316 (2002), 201-211.
7.	W. Z. He, J.-M. Carazo and J.-J. Fernandez, A new phase consistency criterion and its application in electron crystallography, Ultramicroscopy85(2000) 73-91.
8.	W. Z. He, F. H. LI, H. Chen, K. Kawasaki and T. Oikawa, Image deconvolution for defect crystals in field emission high resolution electron microscopy, Ultramicroscopy 70(1997) 1-11.
9.	D. X. HUANG, W. Z. He and F. H. LI, Multi-solution in maximium entropy image deconvolution -- origin and way out,Ultramicroscopy 62(1996) 141-148.